Molecular Imaging

1. IVIS Lumina II (PerkinElmer, USA)

IVIS Lumina II is 2D optical imaging system, both for live cell and live animal imaging in Bioluminescence, Fluorescence and Cherenkov luminescence mode.  It allows scanning of upto 3 mice at a time.  Equipped with four standard fluorescence emission filters GFP, Cy5.5, DsRed and ICG.

Technical Features:

Applications:

IVIS Lumina II is best suited for routine 2D bioluminescence and fluorescence imaging studies:

1. Oncology: To monitor tumor growth, metastasis, and anti-cancer therapies in real time using rodent models (Orthotopic, Syngeneic Tumor Models, and Metastasis models).
2. Gene Expression: To evaluate specific promoter activity and biological process using luciferase reporter genes in vitro.
3. Infectious Diseases: To check the spread, progression, and clearance of viral or bacterial infections using luminescent pathogens.

2. IVIS Spectrum (Revvity, USA formerly PerkinElmer, USA)

IVIS spectrum is used for both live cell and live animal imaging in Bioluminescence, Cerenkov luminescence and NIR fluorescence mode.
 Allow scanning of minimum one to maximum five mice per scan in 2D planar mode and 1 mouse for 3D tomographic imaging mode
 Spectral unmixing imaging capacity of IVIS Spectrum provides scope of using multiple probes emitting non-overlapping photonic signatures.  Uses Structured Light Image to reconstruct the surface tomography of the subject for DLIT and FLIT algorithm.
 Uses transillumination setup to enable effective excitation of deep tissue signal in FLIT mode.

Technical Features:

Applications:

IVIS Spectrum is best suited for routine and advanced multispectral bioluminescence & fluorescence imaging studies:

1. Pharmacokinetics & Pharmacodynamics: To check the efficacy of various forms of therapy interventions, biodistribution and uptake studies using various radio-isotopes and fluorescent probes in whole animal context.
2. Protein-protein interaction: Utilizing bioluminescent reporters to study the activation of specific genes and molecular pathways.
3. Immunology and Stem Cell Research: Observing immune responses, inflammation, and graft-versus-host disease progression longitudinally. Imaging stem cell graft differentiation, testing cell / medical implants in vivo using suitable phantom or model system.

3. IVIS Spectrum CT (Revvity, USA formerly PerkinElmer, USA)

 The IVIS Spectrum-CT is a powerful platform for small animal imaging in pre-clinical settings.
 It provides key optical imaging functionalities: Bioluminescence (BLI), Fluorescence (FLI) and Cerenkov luminescence, for both live cell and live animal imaging.
 High throughput system which allow upto 5 mice in 2D planar scanning mode and upto 2 mouse for 3D scanning mode
 This system provides full 3D tomographic imaging support with co-registered optical signals with precise anatomical (CT) images for volumetric reconstruction of molecular signals.
 Operate in a board spectral range, from visible to near infrared (NIR) light. Spectral unmixing imaging capacity provides scope of using multiple probes emitting non- overlapping photonic signatures.

Technical Features:

Applications:

Common preclinical and biomedical applications include:

1. Oncology: Enables the longitudinal tracking of tumor growth and metastasis over time. The integrated micro CT then provides 3D anatomical maps (e.g., skeletal structures or lung cavities) to pinpoint the exact location of the tumor.
2. Angiogenesis Studies: Tracks the formation of new blood vessels feeding tumor growth using near-infrared (NIR) fluorescent targeted or activatable agents.
3. Bone disease imaging: Combine CT bone anatomy with optical markers of bone remodeling.

• Users are required to submit signed End User Form before starting new experiments. Email the End User at Form to - Email id mifacility[at]actrec[dot]gov[dot]in
• An online booking software is created to book imaging slots. Users can book slots via URL http://10.100.36.253/FacilityEquipBooking by entering last 6 digits of their CC number and password.
• Booking slots open a week before; no more than 2 consecutive time slots can be booked by any user.
• External service users can contact MIF facility directly and discuss requirements.

Committee Members -

• Dr. Abhijit De
  Principal Investigator and Scientific Officer ‘H’
  Email: ade[at]actrec[dot]gov[dot]in
• Dr. Syed Hasan
  Principal Investigator and Scientific Officer ‘F’
  Email: shasan[at]actrec[dot]gov[dot]in

Student Members:
Ms. Diksha Joshi
Mr. Omkar Dhurat

Facility In-charge: Ms. Snehal Valvi
Email: snehal.valvi[at]actrec[dot]gov[dot]in

Facility Staff: Mr. Amandeep Jast
Email: jastaman[dot]actrec[at]gmail[dot]com

Molecular Imaging Facility
AH-105 and 107, First Floor, Laboratory Animal Facility, ACTREC, TMC
Plot No.1 & 2, Sector 22
Kharghar, Navi Mumbai - 410 210.
Contact: 022-27405000 / 022-68735000 and ask for extension 5682
Email: mifacility[at]actrec[dot]gov[dot]in