The Mass Spectrometry facility at ACTREC houses two state-of-the-art mass spectrometry platforms connected to high performance liquid chromatographic systems – one is the Nano-LC (ABSCIEX, Eksigent)-ESI-Q-TOF (ABSCIEX, Triple TOF 5600 plus), and the second one is MALDI-TOF/TOF (Bruker Daltonics, Ultraflex II) equipped with an Agilent 1200 series micro LC system and a spotter (Bruker Daltonics, Proteineer).
After achieving skill and experience of various trials and trouble shooting of sample preparation, sample purification, method development techniques, data analysis parameter settings on the nanoLC-ESI-Q-TOF, the Mass Spectrometry team successfully carried out sample analysis and began accepting samples for study from users. Besides profiling 258 complex protein mixtures, 107 samples were analyzed for the identification of enriched proteins.
The team also successfully performed label free quantification (SWATH analysis) of 90 complex protein samples. Further, 14 samples were analyzed for i-TRAQ label quantification and 20 samples for PTM determination. Demonstration of MALDI TOF-TOF was conducted as and when required for visiting faculty and students.