Toll-like receptors and antitumor immunity: Role of γδ T lymphocytes
Chiplunkar, Paleja (JRF), Tongaonkar, D’Cruz, Kane, Deodhar
 
        The present project aims at understanding the role of Toll like receptors (TLRs) and regulatory T cells in modulating the anti tumor immunity mediated by γδ T cells in patients with oral and cervical cancer. Preliminary work demonstrated the presence of TLRs (TLR2, TLR3, TLR4 and TLR9) on γδ T cells from freshly isolated peripheral blood leucocytes (PBLs). γδ T cells were immunomagnetically purified from ex-vivo expanded PBLs and stimulated in the presence of a TCR stimulus (IPP and anti-CD3) or a TLR stimulus (Poly I:C) or both in combination. Immunophenotyping of stimulated and unstimulated purified γδ T cells showed the presence of TLR2, TLR3, TLR4 and TLR9 on these cells. Further the culture supernatant from anti-CD3 stimulated γδ T cells alongwith Poly I:C showed increased IFN-gamma production. Immunomagnetically purified CD4+CD25+regulatory T cells showed high expression of TLR2 and TLR4. Studies are in progress to characterize the down stream signaling pathways initiated by TLR agonists and further analyze the crosstalk between TLR agonist stimulated γδ T cells and regulatory T cells.
 
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