Dr. Amit Dutt
Principal Investigator, Scientist F
Wellcome Trust/DBT India Alliance Int Fellow
ACTREC, Tata Memorial Centre,
Navi Mumbai,410 210 INDIA
Ph: +91-22-27405056
Email: adutt[at]actrec.gov.in

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The goal of Dutt laboratory is to understand the somatic genetics of human cancer and help develop the next generation of effective targeted therapies to improve treatment of cancer patients. The group specifically focuses on the genomic features of genetic alterations underlying oncogenesis and cancer progression in the lung, breast, cervical, gallbladder, head and neck, and others cancers. The efforts involve using advanced sequencing methodologies followed by functional validation to study the implications of genetic alterations in identifying novel cancer dependencies, therapeutic strategies, and biomarkers.

Cancer Genomics: Using computational approaches we analyze genome wide alterations underlying the cancer genome such as point mutations, complex structural alterations, and aberrant transcript fusion and expression. We develop robust in-house automated computational pipelines and analytical tools to analyze the high throughput datasets generated by whole exome, transcriptome and genome sequencing such as Waterhose, ClinOme, Cancer Pathogen Detector, HPVDetector and others. We recently developed TMC-SNPdb- the first Indian germline SNPdb.






Functional Genomics: The genome-discovery efforts made in the laboratory are paired with functional validation using biochemical, molecular, cell based experimental approaches, and transgenic mouse models. We perturb the candidate target genes using CRISPR-Cas system and perform genome wide pooled shRNA screen using tumor derived cell lines to complement and validate our findings from genomic approaches. Specifically, the lab focusses on EGFR, FGFR and NOTCH family pathways.

Pathogen Discovery: We have developed a computational pipeline, HPVDetector to detect the presence of HPV DNA sequences in human clinical cancer samples. Next we used a similar aproach to detect the presence of non-typhoidal Salmonella in gallbladder cancer. We are currently expanding our endeavor to explore the presence of several other pathogen sequences in the human cancer genome using Cancer Pathogen Detector (CPD), an improved version of the computation pipeline. Simultaneouly, we are in process to deploy the CPD application as a “Container” onto the host cloud system (like CGC) using Docker system.

Pubmed

Dutt Lab in News

   TMC overcomes resistance to hormonal therapy for breast cancer

Researchers at the Tata Memorial Centre (TMC), Mumbai, have found a possible reason why certain breast cancer patients become resistant to hormonal treatment.And the good news is that they have designed an anti-microRNA (small non-coding RNA) which can be probably help in overcoming the resistance to hormonal therapy.
[READ THE ARTICLE HERE]

   Novel mode of action of naturally occurring hormone, Progesterone, in suppressing breast cancer

A randomized clinical study carried out at the Tata Memorial Center in 2011 revealed that mimicking “luteal phase of menstrual cycle” by artificially elevating the levels of progesterone in patients at the time of primary breast cancer surgery was found to be beneficial in terms of survival among those breast cancer patients whose tumor had spread to the nearby lymphatic nodes.
[READ THE ARTICLE HERE]

   Biomarker tells of tongue cancer spread

Researchers at the Tata Memorial Centre in Mumbai have identified a biomarker that will help doctors decide whether patients with early-stage tongue cancer should undergo neck surgery to remove 20-30 lymph nodes. Patients negative for the biomarker can be spared of neck dissection.
[READ THE ARTICLE HERE]

   Genetic signature for tobacco/nut chewing HPV-negative early tongue cancer & prognostic biomarker for metastasis

In a recent report published in Oral Oncology, an official journal of the European Association of Oral Medicine, International Association of Oral Pathologists, we describe that distinct genomic alterations underlie tobacco/ nut chewing, Human papillomavirus (HPV)-negative early stage tongue tumors and identify a gene, Matrix Metalloproteinase (MMP10), as a potential prognostic biomarker to identify those likely to develop cancer metastases (spread of cancer from one organ to another).
[READ THE ARTICLE HERE]

   New genetic marker for oral cancer

By sequencing the genomes of specific oral cancer patients, researchers have identified a marker which is highly expressed in tongue tumour cells1.This gene is a potential biomarker for early-stage tongue cancer.Tongue squamous cell carcinoma is the most predominant form of oral cancer in patients in developing countries.
[READ THE ARTICLE HERE]

   Potential therapy for lung cancer

Researchers have identified novel gene mutations that can lead to lung cancer1. The mutations in a gene that encodes a protein called fibroblast growth factor receptor 3 are potential drug targets for developing new therapies.
[READ THE ARTICLE HERE]

   A new and free computational tool for HPV detection

Human papilloma virus (HPV) accounts for the most common cause of all virus-associated human cancers. However, despite large-scale genome wide DNA sequencing efforts of the cancer genome there is no dedicated informatics tool to rapidly detect the presence of HPV in these genomes, in an exclusive manner.
[READ THE ARTICLE HERE]

   For lung cancer patients in India, key to treatment could be malfunction gene

LUNG CANCER is one of the most common forms of cancer among men in India and worldwide. Cancer being a genetic disease,lung cancer involves alteration of certain genes, leading to unregulated growth of cells in affected body parts.
[READ THE ARTICLE HERE]

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Amit Dutt, Ph.D
Principal Investigator, Scientist F
Wellcome Trust/DBT India Alliance Int Fellow
ACTREC, Tata Memorial Centre
Navi Mumbai, 410 210 INDIA
Ph: +91-22-27405056
Email: adutt[at]actrec.gov.in